method of protecting a plant propagating material, a plant, a part of a plant and / or a plant organ

专利摘要:
patent summary of the invention: "pesticidal compositions". the present invention relates to combinations suitable for agricultural use, which may include (i) a nematode antagonist biocontrol agent and (ii) m or more selected agents, independently of each other, from any one of (a) to (h): (a) at least one fungicide; (b) at least one insecticide; (c) at least one synthetic nematicide; (d) bacteria of the bacillus genus; (e) harpin; (f) isoflavones; (g) plant growth regulators; and / or (h) plant activators.
公开号:BR112013026394B1
申请号:R112013026394
申请日:2012-04-13
公开日:2019-12-24
发明作者:Luiz-Freitas De Oliveira Andre；George Watrin Clifford；Oostendorp Michael；Pedersen Palle
申请人:Syngenta Participations Ag；
IPC主号:

专利说明:

Descriptive Report of the Invention Patent for METHOD OF PROTECTING A PLANT PROPAGATION MATERIAL, A PLANT, A PART OF A PLANT AND / OR A PLANT ORGAN AGAINST PEST DAMAGE.
Technology Area [001] The present description relates in general to the use of nematode antagonist biocontrol agents, and more specifically, with combinations of at least one nematode antagonist biocontrol agent from one or more defined agents. Methods for using these can include, for example, agricultural methods to improve the growth characteristics of a plant. Methods for using these combinations may also include, control of pest damage, such as insects, nematodes and pathogens, especially in agriculture.
Background [002] A variety of active ingredients, agents and combinations are described in the literature to, among others, improve plant growth; however, these known uses do not always satisfy the needs of agricultural practice in many cases and aspects. There is an ongoing need to provide improved combinations, which can provide better, for example biological properties, for example, synergistic properties, especially for pest control. Furthermore, the biological properties of known combinations are not always completely satisfactory in the areas of pest control, phytotoxicity, and the environment and worker exposure, for example. In particular, in the event that a pest has become, or is at risk of becoming resistant to, known compositions or active components, improved methods of control or prevention are sought.
[003] One such pest known to damage crops is the nematode. There are many types of nematode pests, one such nematode is the soy cyst nematode (NCS), Heterodera glycines Ichinohe. NCS causes substantial losses in soy production. The yield suppression attributed to H. glycines alone results in an estimated loss of $ 750 million
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Millions for soybean producers in the US annually from 2003 to 2005 (Wrather, JA, and Koenning, SR, Estimates of disease effects on soybean yields in the United States 2003 to 2005, J. Nematol. 38: 173-180, 2006) .
[004] In light of the methods currently used for pest control, for example, nematode pests, applicants believe that there is still a need for greater pest control. Applicants also want to reduce the rate at which pests acquire an increased tolerance for pest-resistant crops and pesticides. Applicants also want to extend the useful life of crop plants that are resistant to pests and pesticides. Various aspects of the present description can be addressed to at least one of the problems mentioned above, or additional.
Summary [005] As a summary, the present technology includes combinations of at least one antagonistic biocontrol agent for nematodes and one or more defined agents. Defined agents can include agents that provide growth characteristics of a plant through pest control, for example, enabling the availability of nutrients, by activating plant growth properties, for example, natural defense mechanisms, and the like. The disclosed combinations may, in some instances, provide unexpected control or prevention of damage caused by pests in a plant, in which the particular components of the defined combination are applied, in any desired sequence or simultaneously, to the plant, part of a plant, organ plant, and / or in its plant propagation material, or in the area surrounding it.
Consequently, the present description provides, among others, a combination, especially a pesticidal combination, suitable for agricultural use comprising (I) at least one nematode antagonist control agent and (II) one or more selected agents, independently of one another from the others, from either (A) to (H):
(A) at least one fungicide;
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3/49 (B) at least one insecticide;
(C) at least one synthetic nematicide;
(D) bacteria of the genus Bacillus ·, (E) Harpina;
(F) Isoflavones;
(G) Plant growth regulators; and / or (H) Plant activators.
[007] In a second aspect, current technology provides a method of controlling or preventing damage caused by pests in plant propagating material, a plant, a part of a plant and / or plant organ that grow in later on. time, comprising application to the pest, plant, part of the plant, plant organ, plant propagation material or in an area surrounding it, a combination comprising (I) at least one nematode antagonist biocontrol agent and (II) one or more agents selected, independently of each other, from either (A) to (H), in any desired sequence or simultaneously.
[008] In a third aspect, the present technology provides a method of protecting a plant propagating material, a plant, a part of a plant and / or plant organ that grows later in time against damage caused by pests through application to the pest, plant, plant part, plant organ, plant propagation material or an area around it, comprising a combination of (I) at least one nematode antagonist biocontrol agent and (II) one or more agents selected, independently of each other, starting from any one of (A) to (H), in any desired sequence or simultaneously.
[009] In one embodiment, the present technology includes a method of protecting a plant propagating material, a plant, a part of a plant and / or a plant organ that grows later in time against damage caused by pests by applying the combination components to plant propagating material,
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4/49 comprising (I) at least one nematode antagonist control agent and (II) one or more agents selected, independently of each other, starting from any one of (A) to (H), in any desired sequence or simultaneously .
[0010] The present technology also refers to a plant propagation material treated with the combination, comprising (I) at least one nematode antagonist biocontrol agent and (II) one or more selected agents, independently of each other, departing from either (A) to (H), in any desired sequence or simultaneously.
[0011] Furthermore, in an embodiment, the present technology refers to a method comprising (i) treating a plant propagating material, such as a seed, with the combination, comprising (I) at least one biocontrol agent nematode antagonist and (II) one or more selected agents, independently of each other, from either (A) to (H), in any desired sequence or simultaneously, and (ii) planting or sowing the treated propagation material , in which the combination protects against damage caused by pests of the treated plant propagation material, or part of the plant, plant organ and / or cultivated plants starting from treated propagation material.
[0012] Also, in an embodiment of the present technology it refers to a method comprising (i) the treatment of a plant propagating material, such as a seed, with the combination, comprising (I) at least one agent nematode antagonist biocontrol and (II) one or more selected agents, independently of each other, from either (A) to (H), in any desired sequence or simultaneously, and (ii) planting or sowing the treated propagation, and (iii) ensure protection against damage caused by pests of the treated plant propagation material, or part of the plant, plant organ and / or cultivated plants starting from treated propagation material.
[0013] Above, it was intended to summarize certain aspects of the various
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5/49 modalities for carrying out this description. The combinations and methods will be presented in more detail, along with examples demonstrating the effectiveness of the data and the detailed description below. It will be evident, however, that the detailed description is not intended to limit the present invention.
Detailed Description of Exemplary Embodiments [0014] Nematode antagonist biocontrol agents suitable for use in the present technology include nematophagous fungi and nematophagous bacteria. The term nematode antagonist biocontrol agent, as used herein, refers to an organism that inhibits nematode activity, growth or reproduction, or reduces nematode-related disease in plants, or that releases or contains toxic substances or inhibitors of nematodes.
[0015] Nematophagous fungi useful here include, but are not limited to Arthrobotrys spp, for example, Arthrobotrys oligospora, Arthrobotrys superb and Arthrobotrys dactyloides-, Dactylaria spp, for example, Dactylaria Candida ·, Harposporium spp, for example,
Harposporium anguillulae-, Hirsutella spp, for example, Hirsutella rhossiliensis and
Hirsutella minnesotensis, Monacrosporium spp, for example, Monacrosporium cionopagunr, Nematoctonus spp, for example, Nematoctonus geogenius, Nematoctonus leiosporus-,
Meristacrum spp, for example, Meristacrum asterospermunr, Harposporium spp, for example, Harposporium anguillulae-, Paecilomyces spp, for example, Paecilomyces lilacinus-, Pochonia spp, for example, Pochonia chlamydopora and Streptomyces spp.
[0016] Nematophagous bacteria useful in the present invention include, but are not limited to, obligatory parasitic bacteria, opportunistic parasitic bacteria, rhizobacteria, parasporal Cry protein forming bacteria, endophytic bacteria and symbiotic bacteria. In particular embodiments, the biocontrol agent can be a kind of
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6/49 bacteria selected from Actinomycetes spp., Agrobacterium spp., Allorhizobium spp., Arthrobacter spp., Alcaligenes spp., Aureobacterium spp., Azobacter spp., Azorhizobium spp., Azospirillium spp., Beijerinckia spp. Bradyr , Burkholderia spp., Chromobacterium spp., Clavibacterspp., Clostridium spp., Comomonas spp., Corynebacterium spp., Curtobacterium spp., Desulforibtio spp., Enterobacter spp., Flavobacterium spp., Gluconobacter spp., Hydrogenphage ., Methylobacterium spp., Phyllobacterium spp., Phingobacterium spp., Photorhabdus spp., Rhizobium spp., Serratia spp., Stenotrotrophomonas spp., Xenorhadbus spp. Variovorax spp, Pasteuria spp., Pseudomonas spp., And Paenibacillus spp.
Preferred nematode antagonist biocontrol agents include ARF18; Arthrobotrys spp .; Chaetomium spp .; Cylindrocarpon spp .; Exophilia spp .; Fusarium spp .; Gliocladium spp .; Hirsutella spp .; Lecanicillium spp .; Monacrosporium spp .; Myrothecium spp .; Neocosmospora spp .; Paecilomyces spp .; Pochonia spp .; Stagonospora spp .; vesicular-arbuscular mycorrhizal fungi, Burkholderia spp .; Pasteuria spp .; Brevibacillus spp .; Pseudomonas spp .; and Rhizobacteria.
[0018] Particularly preferred nematode antagonist biocontrol agents include ARF18, Arthrobotrys oligospora, Arthrobotrys dactyloides, Chaetomium globosum, Cylindrocarpon heteronema, Exophilia jeanselmei, Exophilia pisciphila, Fusarium Aspergillus, Fusarium, Glarium, Glarium, Melium, Glio Hirsutella minnesotensis, Lecanicillium lecanii, Monacrosporium drechsleri, Monacrosporium gephyropagum, verrucaria Myrotehcium, vasinfecta Neocosmospora, Paecilomyces lilacinus, Pochonia chlamydosporia, Stagonospora heteroderae, Stagonospora phaseoli vesicular-arbuscular mycorrhizal fungi, Burkholderia cepacia, Pasteuria penetrans, Pasteuria thornei, Pasteuria nishizawae, Pasteuria ramosa , Pastrueia usage, G4 strain of Brevibacillus laterosporus, Pseudomonas fluorescens and Rhizobacteria.
[0019] The present technology provides a combination, especially a pesticidal combination, suitable for agricultural use comprising (I) at least one antagonist biocontrol agent of
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7/49 nematodes, described above, and (II) one or more selected agents, independently of each other, starting from any one of (A) to (H).
[0020] Fungicides, (A), suitable for use in the present invention include at least one member selected from the group consisting of amisulbrome, azoxystrobin, boscalide, captana, carboxine, cyproconazole, cyprodinil, diphenoconazole, dimoxystrobin, enestrobin, fludioxonil, fluopyrame, fluoxastrobin, fluquinconazole, flutriafol, fuberidazole, flutriafol, fluxpyroxade, ipconazole, mancozeb, mefenoxam, metalaxyl, myclobutanil, puffufen, pentiopirad, prochloraz, protioconazole, pyraclostrobin, pyraclostrobin, pyraclone -methyl, thiram, triadimenol, triazoxide, trifloxystrobin and triticonazole.
[0021] Insecticides, (B), suitable for use include at least one member selected from the group consisting of thiamethoxam, clothianidin, imidacloprid, thiaclopride, acetamipride, betaciflutrin, cyantraniliprole, diafentiuron, diazinon, emamectina (benzoate), phenol , fipronil, flonicamide, lambda-cyhalothrin, lufenuron, metiocarb, pimetrozine, pyriproxyphene, pyrifluquinazon, espinetorame, spinosad, spirotetramato, teflutrina, tiodicarb or Ti-435.
[0022] Synthetic nematicides, (C), suitable for use in the present invention include at least one member selected from the group consisting of avermectin nematicides, such as abamectin; carbamate nematicides, such as, aldicarb, thiadicarb, carbofuran, carbosulfan, oxamil, aldoxicarb, etoprope, metomil, benomyl, alanicarb and organophosphate nematicides, such as phenamiphos, phensulfothione, terfufos, phosphosphonate, dichloride, phosphate, phosphate Isazophos etoprofos, cadusafos, terbufos, chlorpyrifos, dichlofention, heterofos, isamidofos, mecarfon, forato, thionazine, triazophos, diamidafos, fostietano and phosphamidone
[0023] Bacteria of the genus Bacillus, (D), suitable for use in the present invention include at least one member selected from the group consisting of Bacillus sp B16, Bacillus agri, Bacillus aizawai, Bacillus albolactis, Bacillus amyloliquefaciens, Bacillus cereus, Bacillus
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8/49 firmus, Bacillus coagulans, Bacillus endoparasiticus, Bacillus endorithms, Bacillus firmus, Bacillus kurstaki, Bacillus lacticola, Bacillus lactimorbus, Bacillus lactis, Bacillus laterosporus, Bacillus lentimorbus, Bacillus licheniformis, Bacillus megillus, Bacillus megillus, Bacillus megillus, Bacillus megillus Bacillus nigrificans, Bacillus popillae, Bacillus pumilus, Bacillus siamensis, Bacillus sphaericus, Bacillus spp., Bacillus subtilis, Bacillus thuringiensis (including those forming toxic Cry proteins for nematodes and / or nematode larvae such as Cry5, Cry6, 13 Cry14 and Cry21), Bacillus thuringiensis israelensis; Bacillus thuringiensis kurstaki, Bacillus uniflagellates, in addition to those listed in the Bacillus Genus category in Bergey's Manual of Systematic Bacteriology, First Edition (1986) alone or in combination. In a particularly preferred embodiment, bacteria of the genus Bacillus comprise spores of at least one B. firmus CNCM 1-1582 and / or spores of B. cereus strain CNCM 1-1562 as described in US patent No. 6,406,690, which is incorporated herein by reference in its entirety. In other preferred embodiments, the bacteria are at least one B. amyloliquefaciens IN937a, at least one strain of Bacillus subtilis designation GB03, or at least one strain of B. pumilus designation GB34. Combinations of the four bacterial species listed above, as well as other root-colonizing and spore-forming bacteria known to exhibit agriculturally beneficial properties are within the scope and spirit of the present invention. Particularly preferred embodiments according to the invention are also those compositions which comprise B. firmus CNCM 1-1582 spore mutants and / or B. cereus spore strain CNCM 1-1562. Most particularly preferred are those mutants that have nematicidal activity.
[0024] Harpina (E), (CAS RN 151438-54-9) is a protein produced by the plant's pathogenic bacteria, Erwinia amylovora. It is available under the Messenger ™ brand. It is described in US 5,849,868 and US 5,776,889. Harpina was disclosed as a plant activator, for example, in WO 95/31564. In some cases, Harpina is known to have the
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9/49 nematicidal characteristics and, consequently, in such cases, the present invention also relates to combinations containing Harpina.
[0025] Isoflavones, (F), are preferably formononetine and gengenistein, biocanin A, daidzein, glycitein, hesperitin, naringenin, chalcone, coumarin, Ambiol (2-methyl-4- [dimethylaminomethyl] -5hydroxibenzimidazole), ascorbate and pratorbate and its salts and esters.
[0026] Plant growth regulators (G) include paclobutrazol, trinexapac-ethyl, and gibberellin GA3, GA7 or a mixture of these.
[0027] Plant activators, (H), include acibenzolar-S-methyl and probenazole.
[0028] In an embodiment of any aspect of the invention, the combination is a composition preferably comprising (I) a nematode antagonist biocontrol agent and (II) one or more pesticidal agents selected from (A) amisulbrome , azoxystrobin, boscalide, captana, carboxine, cyproconazole, ciprodinil, diphenoconazole, dimoxystrobin, enestrobin, fludioxonil, fluopyrame, fluoxastrobin, fluquinconazole, flutriafol, fuberidazole, flutriafol, ipconazol, penoxyl, manoxzol, pentoxyazole, manchezzol , pyraclostrobin, pyribencarb, silkxane, SYP-1620, tebuconazole SYPZ048, thiabendazole, thiophanate-methyl, thiram, triadimenol, triazoxide, trifloxystrobin and triticonazole and, optionally, (III) one or more usual formulation aids.
[0029] In an embodiment of any aspect of the invention, the combination is a composition preferably comprising (I) a nematode antagonist control agent and (II) one or more selected from pesticidal agents (B) thiamethoxam, clothianidin, imidacloprid, thiacloprid, acetamipride, beta-cyfluthrin, cyantraniliprole, diafentiuron, diazinon, emamectin, emamectin benzoate, phenoxycarb, fipronil, flonicamide, lambda-chyotropin, pyridine, pyridine, pyridine, pyridine, pyridine, pyridine, pyridine teflutrin, tiodicarb or Ti-435, and optionally (III), one or more formulation aids
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Usual 10/49.
[0030] In an embodiment of any of the aspects of the invention, the combination is a composition preferably comprising (I) a nematode antagonist biocontrol agent and (II) one or more pesticidal agents selected from (C) at least one synthetic nematicide selected from the group consisting of an avermectin, such as abamectin, carbamate nematicides, such as, aldicarb, thiadicarb, carbofuran, carbosulfan, oxamyl, aldoxicarb, etoprop, methyl, benomyl, alanicarb and organophosphate nematicides, such as , fenamiphos, fensulfothione, terbufós, fostiazate, dimetoate, phosfocarb, diclofentiona, isamidofos, fostietano, Isazofos etoprofos, cadusafos, terbufós, chlorpyrifos, diclofentiona, heterofos, isamidofos, mechaphonine, trifanozide, trhaphonine, trhaphonate, trhaphonate, trioxide
[0031] In an embodiment of any aspect of the invention, the combination is a composition preferably comprising (I) a nematode antagonist control agent and (II) (E) harpin and, optionally (III) a or more usual formulation aids.
[0032] In an embodiment of any aspect of the invention, the combination is a composition preferably comprising (I) a nematode biocontrol antagonist agent and (II) (D) Bacillus firmus strain 1-1582, and optionally, (III) one or more usual formulation aids.
[0033] In an embodiment of any aspect of the invention, the combination is a composition preferably comprising (I) a nematode antagonist control agent and (II) one or more agents selected from (F) Formononetine and Genistein and, optionally, (III) one or more usual formulation aids.
[0034] In an embodiment of any aspect of the invention, the combination is a composition preferably comprising (I) a nematode antagonist control agent and (II) one or more agents selected from (G), a plant growth regulator, such as paclobutrazol, trinexapac-ethyl, and gibberellins GA3, GA7
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11/49 or a mixture thereof, and optionally (III) one or more usual formulation aids.
[0035] In an embodiment of any aspect of the invention, the combination is a composition preferably comprising (I) a nematode antagonist control agent and (II) one or more agents selected from (G), a plant activator and, optionally, (III) one or more usual formulation aids.
[0036] A particularly preferred combination is one selected from (I) a nematode antagonist biocontrol agent and (II) a mixture of (B) one or more of thiamethoxam, clothianidin, imidacloprid, cyantraniliprole, lambda-cyhalothrin, spinosad, spirotetramate, teflutrin or tiodicarb, and (A) one or more of azoxystrobin, trifloxystrobin, fluoxastrobin, cyproconazole, diphenoconazole, protioconazole, tebuconazole, triticonazole, fludioxonil, thiabendazole, ipconazol, xylazone, xylazone, xylazone, xylazone, xylazone, xylazone, xylazone, xylazone, xylazone, xylazone.
[0037] In one embodiment the combination is selected starting from (I) a nematode antagonist biocontrol agent and (II) a mixture of (B) thiamethoxam, and (A) one or more of the amisulbrome, azoxystrobin, boscalide, captana, cyproconazole, ciprodinil, diphenoconazole, dimoxystrobin, enestrobin, fludioxonil, fluopyrame, fluoxastrobin, ipconazole, mefenoxam, metalaxyl, myclobutanil, pyrflufen, pentiopirad, protioconazole, pyraclostrobin, pyracyl, pyrolazone , trifloxystrobin, triticonazole.
[0038] In one embodiment, the combination is selected from (I) a nematode antagonist biocontrol agent and (II) a mixture of (C) and abamectin (A) one or more of amisulbrome, azoxystrobin, boscalide, captana ; , SYP-1620, tebuconazole SYP-Z048, thiabendazole, thiophanate-methyl, thiram, triadimenol, triazoxide, triPetition 870180067939, from 06/08/2018, p. 19/67
12/49 floxystrobin and triticonazole.
[0039] In one embodiment, the combination is selected from (I) a nematode antagonist biocontrol agent and (II) a mixture of (B) lambda-cyhalothrin, and (A) one or more of the amisulbrome, azoxystrobin, boscalide, captana, carboxine, cyproconazole, cyprodinil, diphenoconazole, dimoxystrobin, enestrobin, fludioxonil, fluopyrame, fluoxastrobin, fluquinconazole, flutriafol, fuberidazole, flutriafol, ipconazol, penoxyl, manoxoxazole, procoxazol, mopoxyl, moxyl, zinc pyraclostrobin, piribencarb, silkxane, SYP-1620, tebuconazole SYP-Z048, thiabendazole, thiophanate-methyl, thiram, triadimenol, triazoxide, trifloxystrobin and triticonazole.
[0040] In one embodiment, the combination is one selected from (I) a nematode antagonist biocontrol agent and (II) a mixture of (B) teflutrin, and (A) one or more of amisulbrome, azoxystrobin, boscalide , captana, carboxine, cyproconazole, cyprodinil, diphenoconazole, dimoxystrobin, enestrobin, fludioxonil, fluopyrame, fluoxastrobin, fluquinconazole, flutriafol, fuberidazole, flutriafol, ipconazol, mancozeb, mefenoxam, methaxyl, piralaxyl, piralaxyl, procalaxyl, piralaxyl, , silkxane, SYP-1620, tebuconazole SYP-Z048, thiabendazole, thiophanate-methyl, thiram, triadimenol, triazoxide, trifloxystrobin and triticonazole.
[0041] In one embodiment the combination comprises (I) a nematode antagonist control agent and (II) a mixture of (B) thiamethoxam and (B) one or more of lambda-cyhalothrin, teflutrin, fipronil. In another embodiment, the combination comprises (I) a nematode antagonist biocontrol agent and (II) a mixture of (B) thiamethoxam and (B) one or more of lambda-cyhalothrin, teflutrin, fipronil and, optionally, one or more compounds selected from (A) amisulbrome, azoxystrobin, boscalide, captana, carboxine, cyproconazole, cyprodinil, diphenoconazole, dimoxystrobin, enestrobin, fludioxonil, fluopyrame, fluoxastrobin, fluquinconazole, flutriafol, flubia, flubazole, flubidazole, flubidazole, metalaxyl, miclobutanil, penflufen, pentiopirad,
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13/49 prochloraz, protioconazole, pyraclostrobin, pyribencarb, silkxane, SYP-1620, tebuconazole SYP-Z048, thiabendazole, thiophanate-methyl, thiram, triadimenol, triazoxide, trifloxystrobin and triticonazole.
[0042] In one embodiment the combination comprises (I) a nematode antagonist control agent, (B) thiamethoxam, and (C) abamectin.
[0043] In one embodiment the combination comprises (I) a nematode antagonist biocontrol agent, (B) one or more of clothianidin and thiodicarb, and (C) abamectin.
[0044] Examples of preferred mixtures comprise a nematode antagonist biocontrol agent (I) and any of a) - dd):
a) abamectin;
b) acibenzolar-S-methyl;
c) azoxitronin;
d) Bacillus firmus ·,
e) clothianidin;
f) cyproconazole;
g) diphenoconazole;
h) fludioxonil;
i) fluoxastrobin;
j) fluxpyroxade;
k) Harpin;
l) imidaclopride;
m) mefenoxam;
n) metalaxyl;
o) paclobutrazol;
p) penflufen;
q) prochloraz;
r) protioconazole;
s) pyraclostrobin;
t) pirimiphos-methyl;
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14/49
u) silkxane;
v) spinetorame;
w) spinosad;
x) spirotetramato;
y) tebuconazole;
z) teflutrin;
aa) thiamethoxam;
bb) thiabendazole;
cc) trinexapac-ethyl;
dd) triticonazole;
where (I) is selected from the group consisting of Arthrobotrys spp., Chaetomium spp., Hirsutella spp., Pasteuría spp., Pochonia spp., Pseudomonas spp. and Rhizobacteria. Preferably (I) is selected from the group consisting of Hirsutella spp., Paecilomyces spp., Pasteura spp., Pochonia spp., Pseudomonas spp. and Rhizobacteria. Most preferably (I) is selected from the group consisting of Hirsutella rhossiliensis, Hirsutella minnesotensis, Pasteuría penetrans, Pasteuría thornei, Pasteuría nishizawae, Pseudomonas fluorescens and Rhizobacteria.
[0045] Examples of mixtures comprising 3 or more active ingredients include compositions comprising a (I) nematode antagonist biocontrol agent and any of a) - jj):
a) abamectin + thiamethoxam;
b) Bacillus spp. + thiodicarb and / or clothianidin;
c) Bacillus firmus + thiodicarb and / or clothianidin;
d) clothianidin + harpin;
e) clothianidin + imidaclopride;
f) clothianidin + beta-cyfluthrin;
g) clothianidin + fipronil;
h) clothianidin + teflutrin;
i) clothianidin + thiodicarb;
j) cyproconazole + fludioxonil;
k) fludioxonil + azoxystrobin;
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15/49
l) fludioxonil + azoxystrobin + mefenoxam;
m) fludioxonil + azoxystrobin + metalaxyl;
n) fludioxonil + diphenoconazole;
o) fludioxonil + mefenoxam;
p) fludioxonil + metalaxyl;
q) fludioxonol + triticonazole;
r) fluoxastrobin + protioconazole;
s) imidacloprid + fipronil;
t) imidacloprid + harpin;
u) imidacloprid + thiodicarb;
v) imidacloprid + teflutrin;
w) protioconazole + tebuconazole + triazoxide;
x) protioconazole + tebuconazole;
y) silkxane + azoxystrobin;
z) silkxane + fludioxonil;
aa) silkxane + mefenoxam;
bb) silkxane + metalaxyl;
cc) silkxane + thiabendazole;
dd) silkxane + thiamethoxam;
ee) thiabendazole + azoxystrobin;
ff) thiabendazole + mefenoxam;
gg) thiabendazole + metalaxyl;
hh) thiabendazole + thiamethoxam;
ii) thiamefenoxam + thiamethoxam + fludioxonil;
jj) thiamethoxam + metalaxyl + fludioxonil;
where (I) is selected from the group consisting of Arthrobotrys spp., Chaetomium spp., Hirsutella spp., Pasteuría spp., Pochonia spp., Pseudomonas spp. and Rhizobacteria. Preferably (I) is selected from the group consisting of Hirsutella spp., Paecilomyces spp., Pasteura spp., Pochonia spp., Pseudomonas spp. and Rhizobacteria. More preferably (I) is selected from the group consisting of Hirsutella rhossiliensis, Hirsutella minnesotensis, Pasteuría penetrans, Pasteuría thorPetição 870180067939, from 06/08/2018, p. 23/67
16/49 nei, Pasteuria nishizawae, Pseudomonas fluorescens and Rhizobacteria.
[0046] Each combination according to the invention is especially advantageous for the treatment of plant propagation material. Examples of specific combinations covered by the invention include, but are not limited to:
Nematode antagonist biocontrol agent Other agent (s) Hirsutella spp. azoxystrobin, cyproconazole, diphenoconazole, fludioxonil, mefenoxam, metalaxyl, paclobutrazol, protioconazole, silkxane, tebuconazole, thiabendazole and / or triticonazole Hirsutella spp. thiamethoxam, clothianidin, cyantraniliprole, diafentiuron, emamectin, imidadopride, lambda-cyhalothrin, lufenuron, pirimicarb, profenofos, pimetrozina, espinetorame, espinosad, spirotetramato, teflutrina and / or thiodicarb Hirsutella spp. abamectin and / or phostiazate Hirsutella spp. Bacillus spp. Pseudomonas fluorescens azoxystrobin, cyproconazole, diphenoconazole, fludioxonil, mefenoxam, metalaxyl, paclobutrazol, protioconazole, silkxane, tebuconazole, thiabendazole and / or triticonazole Pseudomonas fluorescens thiamethoxam, clothianidin, cyantraniliprole, diafentiuron, emamectin, imidadopride, lambda-cyhalothrin, lufenuron, pirimicarb, profenofos, pimetrozina, espinetorame, espinosad, spirotetramato, teflutrina and / or thiodicarb Pseudomonas fluorescens abamectin and / or phostiazate Pseudomonas fluorescens Bacillus spp. Pasteuria spp. azoxystrobin, cyproconazole, diphenoconazole, fludioxonil, mefenoxam, metalaxyl, paclobutrazol, protioconazole, silkxane, tebuconazole, thiabendazole and / or triticonazole Pasteuria spp. thiamethoxam, clothianidin, cyantraniliprole, diafentiuron, emamectin, imidadopride, lambda-cyhalothrin, lufenuron, pirimicarb, profenofos, pimetrozina, espinetorame, espinosad, spirotetramato, teflutrina and / or thiodicarb
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17/49
Pasteuría spp. abamectin and / or phostiazate Pasteuría spp. Bacillus sp B16 Pasteuría spp. Bacillus agri Pasteuría spp. Bacillus aizawai Pasteuría spp. Bacillus albolactis Pasteuría spp. Bacillus amyloliquefaciens Pasteuría spp. Bacillus cereus Pasteuría spp. Bacillus coagulans Pasteuría spp. Bacillus endoparasiticus Pasteuría spp. Bacillus endorhythmos Pasteuría spp. Bacillus firmus Pasteuría spp. Bacillus kurstaki Pasteuría spp. Bacillus ladicola Pasteuría spp. Bacillus lactimorbus Pasteuría spp. Bacillus lactis Pasteuría spp. Bacillus laterosporus Pasteuría spp. Bacillus lentimorbus Pasteuría spp. Bacillus licheniformis Pasteuría spp. Bacillus megateríum Pasteuría spp. Bacillus medusa Pasteuría spp. Bacillus metiens Pasteuría spp. Bacillus natto Pasteuría spp. Bacillus nigríficans Pasteuría spp. Bacillus papillae Pasteuría spp. Bacillus pumilus Pasteuría spp. Bacillus siamensis Pasteuría spp. Bacillus sphaericus Pasteuría spp. Bacillus subtilis Pasteuría spp. Bacillus thuringiensis Pasteuría spp. Bacillus uniflagellate
[0047] Other examples of specific combinations covered 870180067939, of 06/08/2018, p. 25/67
18/49 by the invention include, but are not limited to:
Nematode antagonist biocontrol agent Other agent (s) Other agent (s) Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam azoxystrobin Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam cyproconazole Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam diphenoconazole Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam fludioxonil Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam mefenoxam and / or metalaxyl Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam paclobutrazol Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam protioconazole Pasteuría spp. clothianidin and / or imidacloprid silkxane Pasteuría spp. thiamethoxam silkxane Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam tebuconazole Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam thiabendazole Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam triticonazole Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam thiamethoxam Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam clothianidin Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam cyantraniliprole Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam diafentiuron Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam emamectin Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam imidacloprid Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam lambda-cyhalothrin Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam lufenuron Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam pirimicarb Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam profenofos Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam pymetrozine Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam spinelame Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam spinosad Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam spirotetramato Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam teflutrin
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19/49
Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam tiodicarb Pasteuría spp. clothianidin and / or imidacloprid abamectin Pasteuría spp thiamethoxam abamectin Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam fostiazato Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam Bacillus spp. Pasteuría spp. clothiadin, imidacloprid and / or thiamethoxam trinexapac-ethyl
[0048] In a preferred embodiment, the combination is in the form of a composition, which further comprises (III) one or more usual formulation aids. In a preferred embodiment, each combination is a composition that is formulated as a premix composition.
[0049] The combinations may demonstrate synergistic activity in relation to the activity of each of the ingredients of the combination. There can be more than one agent, regardless of each combination, starting from (II). The description, therefore, makes an improvement to the technique available.
[0050] To control, prevent or protect and its inflections, within the context of the present invention, means to reduce any unwanted effect, such as attack or infestation, and damage caused by a pest of a plant, a part of the plant propagation material in a level that demonstrates improvement.
[0051] Each combination according to the invention may have advantageous properties for the protection of plants against, for example, (i) pathogens such as phytopathogens, especially fungi, attack or infestation, which results in disease and damage to the plant and / or (ii) attack or damage by insects or nematodes, particularly in the case of plants, the present invention can control or prevent damage caused by pests in a seed, or parts of plants, plant organs and / or plants. In addition, a combination according to the invention, in the absence of pathogens or insects and / or nematode pressure, can improve the growth of a plant.
[0052] These properties are, for example, synergistic actions
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20/49 combinations compared to the individual components of the combination (for example, (I) and (II)), resulting in, for example, less damage caused by pathogenic pests, lower rates of application, or a longer duration of action. In the case of agriculture, the reinforcement actions may present an improvement in the growth characteristics of a plant, for example, greater than expected pest damage control, or greater than expected yield, establishment of seedlings, germination, etc. .
[0053] Improving the growth characteristics (or growth) of a plant can manifest itself in several different ways, but it will typically lead to a better product of the plant. It can, for example, manifest itself in the improvement of the yield and / or vigor of the plant or the quality of the product harvested from the plant, an improvement that may not be linked to pest control, such as fungi, insects and nematodes.
[0054] As used in this document, the term improve the yield of a plant refers to an increase in the yield of a plant product by a measurable amount over the yield of the same plant product produced under the same conditions, but without the application of the method in question. It is preferred that the yield is increased by at least about 0.5%, more preferably, that the increase is at least about 1%, even more preferably, is about 2%, and even more preferably, is about 4% or more. The yield can be expressed in terms of an amount by weight or volume of a plant product on some basis. The basis can be expressed in terms of time, area of growth, weight of plants produced, amount of raw material used, or the like.
[0055] As used in this document, the term improve the vigor of a plant refers to an increase or improvement in the rate of vigor, or of implantation (the number of plants per unit area), or the height of the plant, or the canopy of the plant, or the visual appearance (such as greener leaf color), or the level of rooting, or emergence, or
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21/49 the protein content, or increased tillering, or greater leaf blade, or less death of basal leaves, or stronger tillers, or less need for fertilizer, or less need for seeds, or more productive tillers, or early flowering, or early grain maturity, or less plant tipping (lodging), or increased sprout growth, or early germination, or any combination of these factors, or any other familiar advantages for a person skilled in the art, by a measurable or noticeable amount above of the same factor of the plant produced under the same conditions, but without the application of the method in question.
[0056] When it is said that the present method is capable of improving the yield and / or vigor of a plant, the present method results in an increase in yield, as described above, or the vigor of the plant, as described above, or the yield and vigor of the plant.
[0057] In one embodiment, the application to a plant, or to its propagating material, a combination of nematode antagonist biocontrol agent and any of the thiamethoxam or cyantraniliprole provides greater performance and / or vigor. The application of the combination helps against possible negative field conditions, which expose the plants to various stressors, such as drought and flooding.
[0058] Consequently, the present description also provides a method of enhancing the growth characteristics of a plant, which comprises application to the plant, plant part, and / or plant propagation material, the ingredients of the combination, as defined in the first aspect, in any desired sequence or simultaneously, especially in the absence of pathogenic organisms or pest pressure.
[0059] Combinations of the description can be used in the agricultural sector and fields related to the use for the control or prevention of damage caused by pests, such as insects, nematodes and pathogens.
[0060] Combinations according to the present description, in
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22/49 especially those containing one or more selected pesticide agents (II), independently of each other, starting from (B), (C) and (D), can be effective for pest control, such as pest control selected from Nematoda, Insecta and Arachnid. In this case, the combination can also be applied to the pest to control or prevent damage caused by pests and to protect the desired material (eg plant and part of the plant) from damage caused by pests.
[0061] Particular pests controlled by the compositions of the present technology include those of the Nematoda class, for example, the species of Tylenchus spp., Atylenchus spp., Anguina spp., Rotylenchus spp., Criconema spp., Tylenchulus spp., Paratylenchus spp. , Aphenlenchus spp., Bursaphelenchus spp., Paralongidorus spp., Trichodorus spp., Meloidogyne spp. (for example, Meloidogyne incoginita and Meloidogyne javanica), Heterodera spp. (for example, Heterodera glycines, Heterodera schachtii, Heterodora avenae and Heterodora trifolii), Globodera spp. (for example, Globodera rostochiensis), Radopholus spp. (for example, Radopholus similes), Rotylenchulus spp., Pratylenchus spp. (for example, Pratylenchus neglectans and Pratylenchus penetrans), Aphelenchoides spp., Helicotylenchus spp., Hoplolaimus spp., Paratrichodorus spp., Longidorus spp., Nacobbus spp., Subanguina spp. Belonolaimus spp., Criconemella spp., Criconemoides spp. Ditylenchus spp., Dolichodorus spp., Hemicriconemoides spp., Hemicycliophora spp., Hirschmaniella spp., Hypsoperine spp., Macroposthonia spp., Melinius spp., Punctodera spp., Quinisulcius spp., Scutellonehp Spine. spp.
[0062] In case the combination contains (II), (F) Harpina, this combination is also effective in enhancing the growth of a plant through, for example, activating the natural defense mechanism in the host plant, referred to as resistance systemic acquired disease (SAR), and / or nematode control, such as Meloidogyne spp. (for example, Heterodera spp. (for example, Heterodera glycines, Heterodera schachtii, Heterodora avenae and Heterodora trifolii).
[0063] Isoflavones are chemical substances in plants
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23/49 that occur widely in members of the Legume family and two examples of these are those defined in (II) (E). They are based on a simple diphenolic ring structure as described for example by Carlson et al (1980) Journal of Chromotography, 198, 193-197 and in US Patent No. 7033621, the contents of which are incorporated by reference. Examples of isoflavones include, but are not limited to, genistein, biocanine A, formononetine, daidzein, glycitein, hesperitin, naringenin, chalcone, coumarin, Ambiol (2-methyl-4- [dimethylaminomethyl] -5-hydroxybenzimidazole), ascorbate and pratensein and its salts and esters. The present invention contemplates the use of both naturally occurring and synthetic isoflavone compounds.
[0064] If a combination contains (II) one or more agents (E), that combination is also effective in enhancing the growth of a plant through, for example, better availability of nutrients for the plant, such as water , and sulfates, nitrates, phosphates and metals, improving absorption by the roots.
[0065] The combinations according to the invention offer opportunities to control resistant pests, for example, Plutella spp. as well as to proactively manage insecticide resistance in various pests.
[0066] If a combination contains (II) one or more agents (F) and / or (G), that combination can also be effective in improving the characteristics of the plants. Examples of improved plant characteristics include, but are not limited to, an increase in stem circumference, change in leaf color, early flowering, synchronization of flowering, decrease in bed, control of a plant's crown size, delaying or eliminating linking crops, increasing disease resistance, improving water use / improving water use efficiency, including, but not limited to, less frequent watering and / or watering (showing less plant wilting, plant capacity to rejuvenate after a watering suspension), higher yield, higher quality / healthy aspect of the plant, greater trans
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24/49 portability, reducing damage caused by insects and smaller plant tops. Synchronized flowering is indicated by flowers materializing within 0.5-1 days of each other throughout the entire culture. This combination is particularly well suited for use in plants and plant propagation material, which are transplanted.
[0067] In an embodiment, other agent (s), such as active ingredients (s), can be used with each combination according to the present invention. Therefore, each of the combinations of the present invention can be mixed with, for example, one or more other known pesticides, such as other fungicides, insecticides, nematicides, etc. The use of additional agents, such as other active ingredients, may be due, for example, to broader spectrum control (eg wider variety of pests, diseases, etc.), lower rates, synergy and savings. An expert would understand that a single pesticide active ingredient may have activity in more than one pest control area, for example, a pesticide may have fungicidal, insecticidal and nematicidal activity. Specifically, aldicarb is known for its insecticidal, acaricidal and nematicidal activity, while metam is known for its insecticidal, herbicidal, fungicidal and nematicidal activity, and thiabendazole and captana can provide nematicidal and fungicidal activity.
[0068] Combinations of the description can be formulated for a particular use. Preferably, each combination is formulated to protect cultivated plants or their propagation materials. Therefore, each combination of the invention can be applied to the plant in a conventional manner, such as leaf sprinkling. Advantageously, each of the combinations is formulated for plant propagating material, such as seeds, treatment applications to enhance the growth of a plant derived from the treated material (or seeds), for example, controlling or preventing damage caused by pests and / or pathogens, which occur in agriculture and forestry, and can particularly damage the plant in the early stages of its development.
[0069] Furthermore, the present invention also provides for the application
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25/49 in the soil of the combinations of the invention to control pests in the soil and / or pathogens originating in the soil. Methods of application to the soil can be through any suitable method, which ensures that the combination penetrates the soil, for example, application in a nursery tray, application in the furrow, soaking of the soil, injection into the soil, drip irrigation, application through sprayers or central pivot, incorporation into the ground (wide diffusion or in band) are these methods.
[0070] The benefits of the invention can also be obtained by (i) treating plant propagation material with a combination or (ii) applying to the location where control is desired, usually the planting site, the combination, or both (I) and (ii). In fact, the benefits of the invention can also be obtained by treating the plant propagating material with one or more of the ingredients of the combination, and then applying it to the place where control is desired with the other (s) ) ingredient (s) of the combination.
[0071] The term plant propagating material is understood to mean all the generating parts of the plant, such as seeds, which can be used for the multiplication of the latter and vegetative material of plants such as seedlings and tubers (for example, potatoes ). Therefore, as used in this document, the part of a plant includes propagating material. One can mention, for example, seeds (in the strict sense), roots, fruits, tubers, bulbs, rhizomes, parts of plants. Germinated plants and young plants, intended to be transplanted after germination or after the emergence of the soil, can also be mentioned. These young plants can be protected before transplantation through total or partial immersion treatment.
[0072] Parts of plants and plant organs that grow later are any sections of a plant that develop from plant propagating material, such as a seed. Plant parts, plant organs, and plants can also benefit from the protection against pest damage obtained by applying each combination to the plant propagation material. In an embodiment, it determines
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26/49 of parts of a plant and certain plant organs that grow at a late point in time can also be considered as plant propagation material, in which they themselves can be applied (or treated) with the combination; and consequently, the plant, other parts of the plant and other plant organs that develop from the treated plant parts and treated plant organs can also be beneficial from the protection against pest damage obtained by applying each of the combinations in certain plant parts and certain plant organs.
[0073] Methods for applying or treating pesticidal active ingredients and mixtures thereof in plant propagating material, especially seeds, are known in the art, and include methods of applying coating, covering, pelleting and immersion of the propagating material. Such methods are also applicable to the combinations according to the invention. In a preferred embodiment, the combination is applied or treated in the plant propagation material by a method in a way that germination is not induced; generally, seed immersion induces germination because the moisture content of the resulting seed is too high. Therefore, examples of suitable methods for applying (or treating) a plant propagating material, such as a seed, are the seed coating, seed covering or seed pelleting and the like.
[0074] It is preferred that the plant propagating material is a seed.
[0075] Although it is believed that the present method can be applied to a seed in any physiological state, it is preferred that the seed is in a sufficiently durable state so that it does not incur damage during the treatment process. Typically, the seed will be a seed harvested from the field; removed from the plant; and separated from any ear, stalk, outer bark, and surrounding pulp or other plant material other than seed. The seed will also preferably be biologically stable as long as the treatment does not cause damage
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27/49 biological in the seed. It is believed that the treatment can be applied to the seed at any time between the harvest of the seed and the planting of the seed or during the sowing process (applications directed to the seed). The seed can also be prepared before or after treatment.
[0076] Even the distribution of the ingredients in the combination and adherence of the same to the seeds is desired during the treatment of the propagation material. The treatment could range from a thin film (coating) of the formulation containing the combination, for example, a mixture of active ingredient (s), in a plant propagating material, such as a seed, where the shape and / or original size are recognizable to an intermediate state (such as cover) and then to a thinner film (such as pelletizing, with many layers of different materials (such as vehicles, for example, clays; different formulations, as well as other active ingredients; polymers and dyes) where the original seed size and / or shape is no longer recognizable.
[0077] One aspect of the present invention includes the application of the combinations on the plant propagation material in a specific way, including the placement of the combination ingredients on the entire plant propagation material or only in parts thereof, including in a single side or in a portion of a single side. One skilled in the art would understand these application methods from the description provided in EP954213B1 and WO06 / 112700.
[0078] The combinations described here can also be used to increase the growth of a plant by treating, or applying, a combination according to the present as a tablet or a suitable substrate and placing, or sowing, the treated tablet, or the substrate, next to a plant propagation material. Such techniques are known in the art, particularly EP1124414, W007 / 67042 and W007 / 67044.
[0079] The application of the combinations described herein in plant propagation material also includes the protection of plant propagation material treated with the combination of the present invention, by applying
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28/49 tion of one or more particles containing pesticides, next to seeds treated with pesticide, where the amount of pesticide is such that the seed treated with pesticide and the particles containing the pesticides together contain an Effective Dose of pesticide and the pesticide dose contained in the seed treated with pesticide is less than or equal to the Maximum Non-phytotoxic dose of the pesticide. These techniques are known in the art, particularly in W02005 / 120226.
[0080] The application of the combinations on the seed also includes coatings of controlled release on the seeds, in which the ingredients of the combinations are incorporated in materials that release the ingredients over time. Examples of controlled-release seed treatment technologies are generally known in the art and include polymer films, waxes or other seed coatings, where the ingredients can be incorporated into the controlled-release material or applied between layers of materials, or both .
[0081] The seed can be treated by applying the insecticidal compound (I) and at least one agent (II), in any desired sequence or simultaneously.
[0082] Seed treatment is carried out on an unown seed, and the term unown seed is intended to include the seed in any period between the harvest of the seed and the sowing of the seed in the soil for the purpose of germination and plant growth.
[0083] The treatment of an unplanted seed is not intended to include those practices in which the active ingredient is applied to the soil, but includes any application practice directed at the seed during the planting process.
[0084] Preferably, the treatment occurs before planting the seed in a way that the seed planted has been pre-treated with the combination. In particular, the covering of the seed or pelleting of the seed are preferred in the treatment of the combinations according to the invention. As a result of the treatment, the ingredients in each combination are adhered to the seed and therefore available for
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29/49 ga.
[0085] The treated seeds can be stored, handled, planted and profiled in the same way as any other seed treated with active ingredient.
[0086] Each combination according to the present invention is suitable for plants of the crops of: cereals, such as wheat, barley, rye, oats, rice or sorghum, maize (fodder maize and sugar maize / sweet and field maize), beet, such as sugar beet or fodder, fruit, for example, pomes, stone fruit, walnut or pulp fruit, such as apples, pears, plums, peaches, bananas, almonds, walnuts, pistachios, cherries or red fruits, for example, strawberries, raspberries or blackberries; leguminous crops, such as beans, lentils, peas or soybeans, oil crops such as rapeseed oil, mustard, poppies, olives, sunflower, coconut, castor, cocoa or peanuts; cucurbits such as pumpkins, courgettes, cucumbers and melons, fiber plants such as cotton, flax, hemp or jute, citrus fruits such as oranges, lemons, grapefruits and tangerines, vegetables such as spinach, lettuce, asparagus, cabbage, iceberg lettuce, carrots, onions, tomatoes, paprika, potatoes and pepper; Lauraceae, such as avocado, cinnamon or camphor, and also tobacco, nuts, coffee, eggplants, sugar cane, tea, pepper, vines, hops, the banana family, including bananas, latex plants, lawn, lawn, forage grass , and ornamental plants, such as petunias, geraniums / pelargons, pansies and impatiens, and shrubs, broadleaf and permanentleaf trees, such as conifers. In particular, the combination is suitable for coffee, citrus fruits, stone fruits (especially apples, pears, plums, peaches), nuts (especially almonds and pistachios) and vegetables. In particular, cotton, soybeans, cereals, corn, vegetables and bananas.
[0087] Each of the combinations according to the present invention is particularly suitable for use in maize, cereals (including rice), rapeseed / canola oil, soybeans, cotton, sugar beet, sunflower, potatoes, beans, sorghum, peas, peanuts, bananas, as well as vegetables, such as cabbages, and vegetable fruits.
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30/49 [0088] The term cultures is to be understood as also including cultivation plants that have been transformed using recombinant DNA techniques, which are capable of synthesizing one or more toxins that act selectively, such as if it knows, for example, from toxin-producing bacteria, especially those of the genus Bacillus.
[0089] Toxins that can be expressed by these transgenic plants include, for example, the insecticidal proteins of Bacillus cereus or Bacillus popilliae, or insecticidal proteins of Bacillus thuringiensis, such as δ-endotoxins, for example CrylAb, CrylAc, Cry1F, Cry1Fa2, Cry2Ab, Cry3A, Cry3Bb1 or Cry9C, or vegetative insecticidal proteins (Vip), for example, Vip1, Vip2, Vip3 or Vip3A; or insecticidal proteins from bacteria that colonize nematodes, for example Photorhabdus spp. or Xenorhabdus spp, such as Photorhabdus luminescens, Xenorhabdus nematophilus-, toxins produced by animals, such as scorpion toxins, arachnid toxins, wasp toxins and other insect-specific neurotoxins, toxins produced by fungi, such as Streptomycetes toxins, vegetable lectins, such as pea lectins, barley lectins or snowdrops lectins; agglutinins; proteinase inhibitors, such as trypsin inhibitors, serine protease inhibitors, patatin, cystatin, papain inhibitors, ribosome inactivating proteins (RIP), such as ricin, corn RIP, abrin, lufin, saporin or briodine; steroid metabolism enzymes, such as 3-hydroxy steroididoxidase, ecdysteroid-UDPglycosyl transferase, cholesterol oxidases, ecdysone inhibitors, HMG-COA reductase inhibitors, ion channel blockers, such as sodium channel blockers or calcium, juvenile hormone stearase, diuretic hormone receptors, stilbene synthase, bibenzyl synthase, chitinases and glucanases.
[0090] In the context of the present invention, δ-endotoxins should be understood, for example, CrylAb, CrylAc, Cry1F, Cry1Fa2, Cry2Ab, Cry3A, Cry3Bb1 or Cry9C, or vegetative insecticidal proteins (VIP), for example, Vip1, Vip2, Vip3 or Vip3A, also, expressly, hybrid toxins
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31/49 of, truncated toxins and modified toxins. Hybrid toxins are produced recombinantly by a new combination of different domains of these proteins (see, for example, WO 02/15701). Truncated toxins are known, for example a truncated CrylAb. In the case of modified toxins, one or more amino acids of the naturally occurring toxin are replaced. In these amino acid substitutions, preferably the non-naturally occurring protease recognition sequences are inserted into the toxin, just as, for example, in the case of Cry3A055, a cathepsin G recognition sequence is inserted into a Cry3A toxin (seeWO 03 / 018810).
Examples of such toxins or transgenic plants capable of synthesizing such toxins are disclosed, for example, in EP0374753A, WO 93/07278, WO 95/34656, EP0427529A, EP451878A and WO 03/052073.
[0092] The processes for the preparation of these transgenic plants are generally known to one skilled in the art and are described, for example, in the publications mentioned above. Cryox-type deoxyribonucleic acids and their preparation are known, for example, from WO 95/34656, EP0367474A, EP0401979A and WO 90/13651.
[0093] The toxin contained in transgenic plants gives plant tolerance to harmful insects. These insects can occur in any taxonomic group of insects, but are especially commonly found in beetles (Coleoptera), two-winged insects (Diptera) and butterflies (Lepidoptera).
[0094] Transgenic plants containing one or more genes that encode an insecticidal resistance and express one or more toxins are known and some of them are commercially available. Examples of such plants are: YieldGard® (a corn variety that expresses a CrylAb toxin); YieldGard Rootworm® (corn variety that expresses a Cry3Bb1 toxin); YieldGard Plus® (corn variety that expresses a toxin and a CrylAb Cry3Bb1); Starlink® (corn variety that expresses a Cry9C toxin); Herculex I® corn variety that expresses a Cry1Fa2 toxin and the enzyme phosphinothricin-N-acetyltransferase (PAT) to achieve
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32/49 tolerance to the herbicide glufosinate-ammonium); NuCOTN 33B® (cotton variety that expresses a CrylAc toxin); Bollgard I® (cotton variety that expresses a CrylAc toxin); Bollgard II® (cotton variety that expresses a CrylAc and a Cry2Ab toxin); VipCot® (cotton variety that expresses a Vip3A and a CrylAb toxin); NewLeaf® (potato variety that expresses a Cry3A toxin); NatureGard®, Agrisure® GT Advantage (tolerant to characteristic GA21 glyphosate), Agrisure® CB Advantage (characteristic Bt11 corn borer (CB)) and Protecta®.
[0095] Other examples of such transgenic crops are:
1. Bt11 corn from Syngenta Seeds SAS, Chemin de I'Hobit 27, F31 790 St. Sauveur, France, registration number C / FR / 96/05/10. Genetically modified Zea mays that have been made resistant to the attack of the European corn borer (Ostrinia nubilalis and Sesamia nonagrioides) through the transgenic expression of a truncated CrylAb toxin. Maize Bt11 also transgenically expresses the PAT enzyme to achieve tolerance to the herbicide glufosinate ammonium.
2. Bt176 maize from Syngenta Seeds SAS, Chemin de I'Hobit 27, F-31 790 St. Sauveur, France, registration number C / FR / 96/05/10. Genetically modified Zea mays that has been made resistant to attack by the European corn borer (Ostrinia nubilalise and Sesamia nonagrioides) by the transgenic expression of a CrylAb toxin. Corn bt176 also transgenically expresses the PAT enzyme to obtain tolerance to the herbicide glufosinate ammonium.
3. MIR604 maize from Syngenta Seeds SAS, Chemin de I'Hobit 27, F-31 790 St. Sauveur, France, registration number C / FR / 96/05/10. Maize that has been made resistant to insects by the transgenic expression of a modified Cry3A toxin. This toxin is Cry3A055 modified by inserting a cathepsin-L-protease recognition sequence. The preparation of these transgenic maize plants is described in WO 03/018810.
4. MON 863 maize from Monsanto Europe S.A. 270-272 Avenue de Tervuren, B-1150 Brussels, Belgium, registration number C / DE / 02/9. MON 863 expresses a Cry3Bb1 toxin and is resistant to certain Coleop insects
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33/49 teros.
5. Cotton IPC 531 by Monsanto Europe S.A. 270-272 Avenue de Tervuren, B-1150 Brussels, Belgium, registration number C / ES / 96/02.
6. Corn 1507 from Pioneer Overseas Corporation, Avenue Tedesco, 7 B-1160 Brussels, Belgium, registration number C / NL / 00/10. Corn genetically modified for the expression of the protein Cry 1F to achieve resistance to certain insects Lepidoptera and the protein PAT to achieve tolerance to the herbicide glufosinate ammonium.
7. Maize NK603 χ MON 810 from Monsanto Europe S.A. 270-272 Avenue de Tervuren, B-1150 Brussels, Belgium, registration number C / GB / 02 / M3 / 03. It conventionally consists of hybrid maize varieties by crossing the genetically modified varieties NK603 and MON 810. Maize NK603 x MON 810 transgenically expresses the CP4 EPSPS protein, obtained from the CP4 strain of Agrobacterium sp., Which provides tolerance to the herbicide Roundup® ( contains glyphosate), and also a Cry1 Ab toxin obtained from Bacillus thuringiensis subsp. kurstaki that provides tolerance to certain Lepidoptera, including the European corn borer.
[0096] The weight ratio of any of the two ingredients in each combination is selected to give, for example, the desired synergistic action. In general, the mass ratio would vary depending on the specific ingredient and how many ingredients are present in the combination. Generally, the weight ratio between any two ingredients, regardless of the combination of the present invention, regardless of each other, is 100: 0.1 to 0.1: 100 including from 100: 1 to 1: 100, 99: 1 , 98: 2, 97: 3, 96: 4, 95: 5, 94: 6, 93: 7, 92: 8, 91: 9, 90:10, 89:11, 88:12, 87:13, 86 : 14, 85:15, 84:16, 83:17, 82:18, 81:19, 80:20, 79:21, 78:22, 77:23,
76:24, 75:25, 74:26, 73:27, 72:28, 71:29, 70:30, 69:31, 68:32, 67:33, 66:34,
65:45, 64:46, 63:47, 62:48, 61:49, 60:40, 59:41, 58:42, 57:43, 56:44, 55:45,
54:46, 53:47, 52:48, 51:49, 50:50, 49:51, 48:52, 47:53, 46:54, 45:55, 44:56,
43:57, 42:58, 41:59, 40:60, 39:61, 38:62, 37:63, 36:64, 35:65, 34:66, 33:67,
32:68, 31:69, 30:70, 29:71, 28:72, 27:73, 26:74, 25:75, 24:76, 23:77, 22:78,
21:79, 20:80, 19:81, 18:82, 17:83, 16:84, 15:85, 14:86, 13:87, 12:88, 11:89,
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10:90, 9:91, 8:92, 7:93, 6:94, 5:95, 4:96, 3:97, 2:98, up to 1:99. Preferred mass ratios between any two components of the present invention are from 75: 1 to 1:75, more preferably, 50: 1 to 1:50, especially 25: 1 to 1:25, advantageously 10: 1 to 1:10, such as 5: 1 to 1: 5.
[0097] In an embodiment, the ratio of (I) to (II), where (II) is one or more of (A), is 1:10 to 500: 1, such as 1: 7 up to 400: 1, preferably from 10: 1 to 300: 1, such as 15: 1 to 80: 1, preferably from 20: 1 to 50: 1.
[0098] In an embodiment, the ratio of (I) to (II), where (II) is one or more of (B), is also 1:10 to 10: 1, such as 1: 5 up to 5: 1, preferably 1: 3 to 3: 1.
[0099] In an embodiment, the ratio of (I) to (II), where (II) is harpin (F), is 1000: 1 to 5: 1, such as 300: 1 to 30: 1 , preferably 100: 1 to 40: 1.
[00100] In one embodiment, the ratio of (I) to (II), where (II) is the specific Bacillus fírmus strain (D), is 15: 1 to 1: 3, such as 10: 1 up to 1: 5, preferably 3: 1 to 1: 1.
[00101] In an embodiment, the ratio of (I) to (II), where (II) is Formononetine, is 5: 1 to 1: 5, such as from 1: 1 to 10: 1, from preferably 3: 1 to 5: 1.
[00102] In an embodiment, the ratio of (I) to (II), where (II) is genistein, is 1: 1000 to 1: 150,000, as 1: 10,000 to 1: 100000, preferably 1: 50000 to 1: 80000.
[00103] In one embodiment, the ratio of (I) to (II), where (II) is a plant growth regulator, is 1:10 to 10: 1, such as 1: 5 to 5 : 1, preferably 1: 3 to 3: 1.
[00104] In one embodiment, the ratio of (I) to (II), where (II) is a plant activator, is 1:10 to 10: 1, such as 1: 5 to 5: 1, preferably 1: 3 to 3: 1.
[00105] In one embodiment, in case (II) is thiamethoxam, the ratio of (I) to (II) is 1: 5 to 5: 1, such as 2 to 5: 1.
[00106] Application rates (use) of a combination
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35/49 varies, for example, according to the type of use, type of culture, the specific agent (II) in the combination, the type of plant propagation material (if appropriate), but it is such that the active ingredients in the combination they are in an effective amount to provide the desired enhanced action (such as disease or pest control) and can be determined through routine testing and experimentation known to those of ordinary skill in the art.
[00107] Generally, for foliar or soil treatments, application rates can vary from 0.05 to 3 kg per hectare (g / ha) of ingredients.
[00108] Generally for seed treatments, application rates can vary from 0.5 to 1,000 g / 100 kg of ingredient seeds. In one embodiment, compound (I) is applied at a rate of 0.01 to 2, preferably 0.03 to 1.5, mg ai / seed, depending on the culture. Considering that the application rates of the agents indicated in (A), (B), (C), (D) and (E) may vary, depending on the specific agent and the type of cultures. These fees would be readily available to one skilled in the art.
[00109] The methods according to the invention for pest control of the type mentioned above are carried out in a manner known per se to those skilled in the art, depending on the intended objectives and prevailing circumstances, which is like saying by spraying, moistening , atomization, brushing, seed coating, dispersion or leakage of the composition. In the case of spore-forming bacteria and fungi, application rates for plant propagation material (eg seed treatment) preferably range from about 1 x 10 ⁵ to 1 x 10 ¹² (or more) spores / seed. Preferably, the spore concentration is about 1 x 10 ⁶ to about 1 x 10 ¹¹ spores / seed. Similar application rates can also be used for agents listed in (D). For example, Bacillus species may be present in the range of about 1 x 10 ⁵ to 1 x 10 ¹² (or more) colony forming units (UFC) / seed. In another example,
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36/49 the concentration of cells is in the range of about 1 x 10 ⁶ to about 1 x 10 ¹¹ CFU / seed.
[00110] The plant propagation material treated by each combination of the present description can therefore be resistant to damage caused by pests; consequently, the present invention also provides a pest resistant plant propagation material, which is treated with each combination, and therefore at least its ingredients are adherent to the propagation material, such as seeds.
[00111] Seed treatment combinations and compositions can also comprise or can be applied together and / or sequentially with other active compounds. These other useful active compounds can be fertilizers or micronutrient donors (such as Mo, Zn and / or Co), or other preparations that influence plant growth, such as inoculants (for example, a nitrogen-fixing bacteria strain), plant inducers (for example, nod factors as described in US2005187107).
[00112] In a preferred embodiment of the invention, soybean seeds and transgenic soybean seeds are treated with a combination of the present invention. In addition, soybean seeds can be inoculated with an appropriate strain of nitrogen-fixing bacteria in order to promote plant growth. Preferably, the seeds can be inoculated with an effective bacterial strain such as Rhizobium spp. or Azospirillium spp. before sowing. The main effect of such bacteria is the fixation of atmospheric nitrogen in a usable form for the plant. Rhizobia bacteria, for example, are especially preferred to form nodules in the roots of plants that are supported by the plant and in turn provide nitrogen for the plant, as mentioned above.
[00113] In another embodiment, a propagating material from the soy plant is treated with a plant inducer, for example, a nod factor derived from Bradyrhizobium japonicum, Sinorhizobium fredii, Sinorhizo
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37/49 bium meliloti, Bradyrhizobium sp. (Arachis), or Rhizobium leguminosarum biovar phaseoli, viceae or trifolii.
[00114] In one aspect, the present invention also provides for the use of the combinations of the present invention, with glyphosate-tolerant plants, especially glyphosate-tolerant soybean plants, in particular for the control of Asian soybean rust. Accordingly, the present invention provides a method comprising: (a) applying a combination according to the invention, as defined in the first aspect, especially those containing (II) one or more pesticidal agents (A), for a material of propagation of glyphosate-tolerant plants, preferably soy propagating material, and (β) application of a pesticidal composition (B) to the resulting plant, a part of the plant and / or the location of it one or more times (i) before of the emergency, (ii) after the emergency, or (iii) both (i) and (ii), provided that the pesticidal composition (B) comprises glyphosate.
[00115] Generally, the composition containing glyphosate can be applied, if applied only once, at a rate of 960 g ai / ha; if applied twice the rate can vary from 1200 to 1680 g ai / ha. Rates and the number of applications vary according to the particular conditions. Preferably, composition (B) is applied three times, with an application rate of 960, 720 and 400 g ai / ha, respectively. In such an embodiment, the present invention controls, prevents or treats Phakopsora pachyrhizi and / or P. meibomiae, especially Phakopsora pachyrhizi.
[00116] Each of the combinations of the present invention can also comprise an alkali metal, alkaline earth metal, metal or ammonium salt. Zinc chloride and alkali metal, alkaline earth metal, or ammonium salts of mineral acids, especially nitrates, phosphates, sulfates, chlorides and carbonates of sodium, potassium, ammonium, magnesium and calcium are preferred.
[00117] Depending on the particular propagation material of the plant to be treated, the conditions under which it is to be stored, and the conditions of the soil and the time under which it is expected to germinate and grow,
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38/49 the combinations of the present invention can include a wide spectrum of one or more additives. These additives include, but are not limited to, UV protectors, pigments, dyes, extenders such as flour, dispersing agents, excipients, antifreeze agents, preservatives, phyto-herbicidal protectors, seed protectors, seed conditioners, micronutrients, fertilizers, agents biocontrol, surfactants, sequestering agents, plasticizers, dyes, brighteners, emulsifiers, flow agents such as calcium stearate, talc and vermiculite, coalescing agents, antifoam agents, humectants, thickeners, waxes, bactericides, insecticides, pesticides, and fillers such as cellulose, glass fibers, clay, kaolin, talc, powdered tree bark (for example, Douglas pine bark or alder bark), calcium carbonate and wood flour, and odor modifying agents. Typical excipients include finely divided mineral substances, such as pumice, atapulgite, bentonite, kaolin zeolite, diatomaceous earth, and other clays, modified diatom adsorbents, coal, vermiculite, finely divided organic substances, such as moss peat, wood dust, and the like. Such additives are commercially available and are known in the art.
[00118] The insecticidal compound (I) and one or more agents (II) and, optionally, any other pesticides, can be used either in its pure form, that is, as a solid active ingredient, for example, in a size of specific particle, or, preferably, together with at least one of the auxiliaries (also known as adjuvants) customary in formulation technology, such as extenders, for example, solvents or solid vehicles, or superficially surfactant compounds, in the form of a formulation, in the present invention. Generally, the insecticidal compound (I) and one or more agents (II) are in the form of a formulation composition with one or more usual formulation aids.
[00119] Therefore, each combination of insecticidal compound (I) and one or more agents (II) is normally used in the form of formulations. The ingredients in the combination can be applied to the location where
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39/49 the control is desired, simultaneously or in succession in a short period of time, for example on the same day, if desired together with other vehicles, surfactants or other adjuvants promoting the application usually employed in the formulation technology. In a preferred embodiment, the ingredients of the combination are applied simultaneously.
[00120] If the ingredients of the combinations are applied simultaneously in the present invention, they can be applied as a composition containing a combination, in which case each of (I) and (II) can be obtained from a separate formulation source and mixed together (known as a ready-to-apply tank mix, spray broth, or suspension), optionally with other pesticides, or (I) and (II) can be obtained as the only formulation mix source (known as a premix, concentrate, formulated product), and optionally mixed together with other pesticides.
[00121] In one embodiment, each combination of the present invention is applied as a composition. Accordingly, the present invention includes a composition comprising (I) and (II) and, optionally, other pesticides and optionally one or more usual formulation aids; which can be in the form of a tank mix or premix composition.
[00122] In one embodiment, each combination of (I) and (II) with one or more usual formulation aids is provided in the form of a premix composition (or a formulated product).
[00123] Alternatively to the current synergistic action in relation to the pesticidal activity, the combinations according to the invention can also have surprising advantageous properties which can also be described, in a broader sense, as synergistic activity. Examples of such advantageous properties that can be mentioned are the following: advantageous behavior during formulation and / or on request, for example, by grinding, sifting, emulsifiers, dissolving or dispensing; greater stability during storage, improved stability
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40/49 lightness; more advantageous degradability; improved and toxicological / or ecotoxicological behavior, or any other familiar advantages for one skilled in the art.
[00124] Examples of foliar formulation types for premix compositions are:
GR: Granules
WP: wettable powders
WG: dispersible water granules (powder)
SG: water soluble granules
SL: soluble concentrates
EC: emulsifiable concentrate
EW: oil-in-water emulsions
ME: microemulsion
SC: concentrated aqueous suspension
CS: aqueous suspension of capsules
OD: oil-based suspension concentrate, and
SE: aqueous suspoemulsion [00125] Whereas, examples of types of pre-mix compositions for seed treatment are:
WS: wettable powders for seed treatment slurry
LS: seed treatment solution
ES: seed treatment emulsions
FS: suspension concentrate for seed treatment WG: granules dispersible in water, and
CS: aqueous suspension of capsules.
[00126] Examples of suitable formulation types for tank mix compositions are solutions, diluted emulsions, suspensions, or a mixture thereof, and dust.
[00127] As with the nature of the formulations, the application methods, such as foliar, submersion, spraying, atomization, dusting, spreading, coating or pouring, are chosen according to the intended objectives and the prevailing circumstances.
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41/49 [00128] Tank mix compositions are generally prepared by diluting with a solvent (for example, water) one or more premix compositions containing different pesticides and, optionally, other auxiliaries.
[00129] Suitable adjuvants and vehicles can be solid or liquid and are the substances commonly used in the formulation technology, for example, regenerated or natural mineral substances, solvents, dispersants, humidifying agents, tackiness agents, thickeners, binders or fertilizers.
[00130] Formulations are prepared in known ways, for example, by homogeneously mixing and / or grinding active ingredients with extenders, for example, solvents, solid vehicles and, where appropriate, active surface compounds (surfactants) ).
[00131] Suitable solvents are: aromatic hydrocarbons, preferably fractions containing 8 to 12 carbon atoms, for example mixtures of xylene or substituted naphthalenes, phthalates, such as dibutyl phthalate or dioctyl phthalate, aliphatic hydrocarbons, such as hexane cycle or paraffins, alcohols and glycols and their ethers and esters, such as ethanol, ethylene glycol, monomethyl or monoethyl ethyl ether, ketones, such as cyclohexanone, strongly polar solvents, such as N-methyl-2pyrrolidone, dimethyl sulfoxide or dimethylformamide , as well as vegetable oils or epoxidized vegetable oils, such as coconut oil or epoxidized soybean oil; or water.
[00132] Solid vehicles used, for example, for dust and dispersible powders, are normally natural mineral fillers, such as calcite, talc, kaolin, montmorillonite or atapulgite. In order to improve the physical properties it is also possible to add highly dispersed salicylic acid or highly dispersed absorbent polymers. Suitable granulated absorbable vehicles are of porous types, for example, pumice, broken brick, sepiolite or bentonite, and suitable non-sorbent vehicles are, for example, calcite or sand. In addition, a large number of pre-granulated materials of an organic or inorganic nature can be used,
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42/49 for example, especially dolomite or pulverized plant residues.
[00133] Depending on the nature of the ingredients to be formulated, the appropriate surfactant compounds are non-ionic, cationic and / or anionic having good emulsifying, dispersing and wetting properties. The term surfactants will also be understood as comprising mixtures of surfactants.
Particularly advantageous application-promoting adjuvants are also natural or synthetic phospholipids from the cephaline and lecithin series, for example, phosphatidylethanolamine, phosphatidylserine, phosphatidylglycerol and lysolecithin.
[00135] Generally, a tank mix formulation for foliar or soil application includes 0.1 to 20%, especially 0.1 to 15%, of the desired ingredients, and 99.9 to 80%, especially 99.9 to 85%, of a solid or liquid auxiliary (including, for example, a solvent such as water), where the auxiliaries can be a surfactant in an amount from 0 to 20%, especially from 0.1 to 15%, with based on the tank mix formulation.
[00136] Typically, a premix formulation for foliar application comprises 0.1 to 99.9%, especially 1 to 95%, of the desired ingredients, and 99.9 to 0.1%, especially 99 to 5% , of a solid or liquid adjuvant (including, for example, a solvent such as water), where auxiliaries can be a surfactant in an amount of 0 to 50%, especially 0.5 to 40%, based on the formulation pre-mix.
[00137] Typically, a mixture tank formulation for applying a seed treatment comprises 0.25 to 80%, especially 1 to 75%, of the desired ingredients and 99.75 to 20%, especially 99 to 25%, of a solid or liquid auxiliary (including, for example, a solvent such as water), where the auxiliaries can be a surfactant in an amount of 0 to 40%, especially 0.5 to 30%, based on the tank formulation mixing.
[00138] Typically, a premix formulation for application in seed treatment comprises 0.5 to 99.9%, especially
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43/49 to 95%, the desired ingredients, and 99.5 to 0.1%, especially 99 to 5%, of a solid or liquid adjuvant (including, for example, a solvent such as water), where the auxiliaries can be a surfactant in an amount of 0 to 50%, especially 0.5 to 40%, based on the premix formulation.
[00139] While commercial products will preferably be formulated as concentrates (for example, premix composition (formulation)), the end user will normally employ diluted formulations (for example, tank mix composition).
[00140] Preferred premix formulations for seed treatment are concentrated in aqueous suspension. The formulation can be applied to seeds using conventional treatment techniques and machines, such as fluidized bed techniques, the roller mill method, roto-static seed scrubbers, and drum coaters. Other methods, such as gushing beds, may also be useful. The seeds can be pre-sized before coating. After coating, the seeds are typically dried and then transferred to a sizing machine for sizing. Such procedures are known in the art.
[00141] In general, the premixed compositions of the invention contain 0.5 to 99.9 especially 1 to 95, advantageously 1 to 50% by weight of the desired ingredients and 99.5 to 0.1 , especially from 99 to 5% by weight of a solid or liquid adjuvant (including, for example, a solvent such as water), where auxiliaries (or adjuvant) can be a surfactant in an amount of 0 to 50 , especially 0.5 to 40%, by weight, based on the mass of the premix formulation.
[00142] A preferred embodiment is a treatment (or protection) composition of plant propagating material, wherein said protection composition of plant propagating material further comprises a coloring agent. The protective composition of plant propagation material or mixture may also comprise at least one polymer starting from pe-forming polymers
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44/49 water-soluble and water-dispersible particles that improve the adhesion of active ingredients to treated plant propagation material, which generally has a polymer of average molecular weight of at least 10,000 to about 100,000.
[00143] The following Examples serve to illustrate the various aspects of the description.
Formulation Examples
Wettable powders The) B) ç) active ingredients 25% 50% 75% sodium lignosulfonate 5% 5% - Sodium lauryl sulfate 3% - 5% sodium diisobutylnaphthalenesulfonate - 6% 10% polyethylene glycol phenol ether - 2% - (7-8 moles of ethylene oxide) highly dispersed silicic acid 5% 10% 10% Kaolin 62% 27% -
[00144] The combination is completely mixed with the adjuvants and the mixture is carefully ground in a suitable mill, providing wettable powders that can be diluted with water to give suspensions of the desired concentration.
Dry treatment powders The) B) ç) active ingredients 25% 50% 75% light mineral oil 5% 5% 5% highly dispersed silicic acid 5% 5% - Kaolin 65% 40% - Baby powder -20
[00145] The combination is completely mixed with the adjuvants and the mixture is carefully ground in a suitable mill, providing powders that can be used directly for seed treatment.
Emulsifiable concentrate
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active ingredients 10% polyethylene glycol octylphenol ether 3% (4 to 5 moles of ethylene oxide)calcium dodecylbenzenesulfonate 3% polyglycolic ether of castor oil (35 mois of ethylene oxide) 4% Cyclohexanone 30% mixture of xylenes 50%
[00146] Emulsions of any necessary dilution, which can be used in plant protection, can be obtained from this concentrate by dilution with water.
Post The) B) Ç) Active ingredients 5% 6% 4% Baby powder 95% - - Kaolin - 94% - mineral charge - - 96%
[00147] Ready-to-use powders are obtained by mixing the combination with the vehicle and grinding the mixture in a suitable mill. These powders can also be used for dry seed coating.
Extruder granulesActive ingredients 15% sodium lignosulfonate 2% carboxymethylcellulose 1% Kaolin 82%
[00148] The combination is mixed and ground with the adjuvants, and the mixture is moistened with water. The mixture is extruded and then dried in an air stream.
Coated granulesActive ingredients 8% polyethylene glycol (200 mol weight) 3% Kaolin 89%
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46/49 [00149] The finely ground combination is uniformly applied, in a mixer, to kaolin moistened with polyethylene glycol. Non-powdered coated granules are obtained in this way.
Suspension concentrateactive ingredients 40% propylene glycol 10% polyethylene glycol nonylphenol ether (15 mois of ethylene oxide) 6% Sodium lignosulfonate 10% carboxymethylcellulose 1% silicone oil (in the form of a 75% water emulsion) 1% Water 32%
[00150] The finely ground combination is intimately mixed with the adjuvants, giving a suspension concentrate from which suspensions of any desired dilution can be obtained by diluting with water. Using these dilutions, live plants as well as plant propagation material can be treated and protected against infestation by microorganisms, by spraying, spilling or immersion.
Flowable concentrate for seed treatmentactive ingredients 40% propylene glycol 5% butanol PO / EO copolymer 2% Triestirenophenol with 10-20 moles of EO 2% 1,2-benzisothiazolin-3-one (as a 20% solution in water) 0.5% monoazo pigment calcium salt 5% Silicone oil (in the form of a 75% water emulsion) 0.2% Water 45.3%
[00151] The finely ground combination is intimately mixed with the adjuvants, giving a suspension concentrate from which suspensions of any desired dilution can be obtained by diluting with water. Using these dilutions, live plants as well as plant propagation material can be treated and protected against infestation.
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47/49 by microorganisms, by spraying, spilling or immersion.
Suspension for Slow Release Capsules [00152] 28 parts of a combination are mixed with 2 parts of an aromatic solvent and 7 parts of the toluene diisocyanate / polymethylene-polyphenylisocyanate mixture (8: 1). This mixture is emulsified in a mixture of 1.2 parts of polyvinyl alcohol, 0.05 part of an antifoaming agent and 51.6 parts of water until the desired particle size is reached. To this emulsion is added a mixture of 2.8 parts of 1,6-diaminohexane in 5.3 parts of water. The mixture is stirred until the polymerization reaction is completed. The obtained capsule suspension is stabilized by adding 0.25 part of a thickener and 3 parts of a dispersing agent. The suspension formulation for capsules contains 28% of the active ingredients. The average diameter of the capsules is 8 to 15 microns. The resulting formulation is applied to the seeds as an aqueous suspension in an apparatus suitable for that purpose.
[00153] Using these formulations, simple or diluted, the plant propagation material can be treated and protected against damage caused by pests, for example, of pathogen (s), through, for example, spraying, spilling or immersion.
[00154] The combinations according to the invention are distinguished by the fact that they are especially well tolerated by plants and are environmentally friendly.
Combination Examples [00155] Combination examples of a plug measuring about 1.9 centimeters (0.75 inches) in diameter and approximately 2.5 cm (1 inch) in depth have been removed from each of the plastic vessels in diameter approx. 7.6 x 20cm (3 inches x 8 inches) deep. Five thousand Heterodera glycines race 3 eggs were placed at the bottom of the plug hole. A variety of 3.6-B6 soybean seed was placed on top of the inoculum. Six repetitions were performed for each treatment group. In addition to the untreated control, the treatment groups were Pasteuría nishizawae, Bacillus firmus, and Pasteuría nishizawae +
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Bacillus firmus.
[00156] Bacillus firmus was applied as a seed treatment (commercially available, for example, from Bayer Crop Science as a VOTIVO, PONCHO / VOTIVO product, with Bacillus firmus in a concentration of at least 2 x 10 ⁹ cfu / ml + clothianidin ( 0.13 mg / seed)). An application point containing Pasteuria nishizawae (approximately 5.0 x 10 ⁹ spores) was applied directly to the seed in the pot. The plug was replaced in the hole to cover the seeds. The soil was superior soil Vero Beach (Florida, USA), pH 5.9 (composition approximately 93% sand, 2% silt, 2% clay, 3% organic matter).
[00157] At 28 days after planting, the plant height was measured and the plants were removed from the pots. The soil was washed gently from the roots. The washed roots were placed in a 20 mesh sieve over a 60 mesh sieve. The root system was blasted with water to remove the nematode females from the cyst. The 20 mesh sieve was eliminated and the cysts were washed to the bottom of the 60 mesh sieve. The cysts were washed into a counting tube under a stereomicroscope. Later, the dry root weights were measured.
[00158] This example illustrates an example of a combination according to a description mode. The results were as follows:
Treatment component (s) Average data points for all repetitions Height (cm) Cysts count Root weight (g) Untreated Control 14.3 375 0.183 Pasteuria nishizawae 13.5 366 0.245 Bacillus firmus 13.7 304 0.168 Pasteuria nishizawae + Bacillus firmus 12.5 81 0.164
[00159] Looking closely at the cysts count as a
nemal control indicator the data show: Treatment components Average Cysts Count % of controlObserved Expected ColbyControl Untreated Control 375 0.00 -
Petition 870180067939, of 06/08/2018, p. 56/67
49/49
Pasteuria nishizawae 366 2.40 - Bacillus firmus 304 18.93 - Pasteuria nishizawae + Bacillus firmus 81 78.40 20.88
[00160] As shown, the expected control for the combination of the Pasteuría + Bacillus species was 20.88%, however, 78.40% of control was observed, showing a strong synergistic effect of the combination.
[00161] In a preferred embodiment, each of the combinations of the present invention is a composition for treating plant propagation material, preferably seeds.
[00162] In each aspect and modality of the invention, "which essentially consist" and inflections thereof are a preferred modality "comprising" and inflections thereof, and "consisting of" and inflections thereof are a preferred modality of "that essentially consists of ”and inflections of it.
[00163] The use of a term in a singular way encompasses that term in the plural and vice versa.

权利要求:
Claims (2)
[1]
1. Method of protecting a plant propagating material, a plant, a part of a plant and / or a plant organ growing at a later point in time against damage from pests, the method characterized by the fact that it comprises :
application to plant propagating material, which is a seed, of a combination comprising (I) Pasteuria nishizawae applied to the seed at a rate in the range of 1 x 10 ⁵ to 1 x 10 ¹² spores / seed; and (II) Bacillus firmus applied to the seed at a rate in the range of 1 x 10 ⁵ to 1 x 10 ¹² CFU / seed plus an insecticide, simultaneously.
[2]
2. Method, according to claim 1, characterized by the fact that it also includes the application to the plant propagation material of the clothianidin insecticide.

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法律状态:
2018-04-03| B06F| Objections, documents and/or translations needed after an examination request according art. 34 industrial property law|

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2018-05-08| B07A| Technical examination (opinion): publication of technical examination (opinion)|

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2019-04-09| B06A| Notification to applicant to reply to the report for non-patentability or inadequacy of the application according art. 36 industrial patent law|

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2019-10-15| B09A| Decision: intention to grant|

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2019-12-24| B16A| Patent or certificate of addition of invention granted|Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 13/04/2012, OBSERVADAS AS CONDICOES LEGAIS. |

优先权:

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申请号 | 申请日 | 专利标题

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US201161475863P| true| 2011-04-15|2011-04-15|

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PCT/EP2012/056806|WO2012140212A2|2011-04-15|2012-04-13|Pesticidal compositions|

---